A Zn_Ta Nano with Dual Inhibitory and Pseudo-Peroxidase Activity toward Lepidium draba Peroxidase

Authors

  • Dorna Dehghani Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
  • Ali Riahi Madvar Department of Molecular and Cell Biology, Faculty of Basic Sciences, Kosar University of Bojnord, Bojnord, Iran
  • Mojtaba Mortazavi
  • Masoud Torkzadeh Mahani Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
  • Qasim Sargazi Non-communicable diseases research center, Bam University of Medical Sciences

DOI:

https://doi.org/10.22034/LSSJ.2026.203

Keywords:

Zn_Ta Nanocomposite, Lepidium draba, Nanozyme, Peroxidase Activity, Molecular Docking

Abstract

Background: Peroxidases play an important role in plant oxidative metabolism and are increasingly explored for biotechnological applications. Nanocomposites containing transition metals may influence enzyme activity and may also exhibit enzyme‑mimicking properties.

Methods: In this study, the effects of a Zn-Ta nanocomposite on the oxidative activity of Lepidium draba peroxidase were investigated using enzyme activity assays complemented by molecular docking analyses. Enzymatic activity was evaluated across different nanocomposite concentrations, and the catalytic properties of the nanocomposite as a pseudo‑peroxidase system were also assessed under varying temperature and pH conditions.

Results: Zinc was associated with an increase in enzyme activity, likely through interactions with the enzyme active site, whereas tantalum showed an inhibitory effect. When combined within the Zn-Ta nanocomposite, an overall reduction in enzyme activity was observed. The highest enzymatic activity was recorded at an optimal concentration of 0.556 mg/mL. In addition, the Zn-Ta nanocomposite exhibited intrinsic pseudo‑peroxidase activity, reaching maximum catalytic performance at 55 °C (pH 3.5) and 70 °C (pH 2.5). Molecular docking analyses indicated interactions among Zn, TMB, H₂O₂, and the enzyme, supporting the activity‑enhancing role of zinc and the inhibitory influence of tantalum.

Conclusion: The Zn-Ta nanocomposite demonstrates dual behavior, functioning both as an inhibitor of Lepidium draba peroxidase and as a pseudo‑peroxidase catalytic system. These findings suggest potential applications of this nanocomposite in biotechnology and diagnostic platforms.

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Published

2026-08-24

How to Cite

Dehghani, D., Riahi Madvar , A. ., Mortazavi, M. ., Torkzadeh Mahani, M., & Sargazi, Q. . (2026). A Zn_Ta Nano with Dual Inhibitory and Pseudo-Peroxidase Activity toward Lepidium draba Peroxidase. Life Sciences Student Journal, 4(2), 1–10. https://doi.org/10.22034/LSSJ.2026.203

Issue

Vol. 4 No. 2 (2026): Vol. 4 No. 2(2026)

Section

Articles

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