The Role of miR-142 and miR-195 in Regulating Apoptotic Pathways in Non-Small Cell Lung Cancer

Authors

  • Alma Davarnia Department of Cellular and Molecular Biology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  • Yasaman Kharratian Department of Cellular and Molecular Biology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  • Mohammad Reza Nourani Tissue Engineering and Regenerative Medicine Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

DOI:

https://doi.org/10.22034/LSSJ.2025.130

Keywords:

Apoptotic Pathways, Lung Cancer, miR-142, miR-195

Abstract

Lung cancer remains the leading cause of cancer-related mortality worldwide. non-small cell lung cancer (NSCLC) accounts for the majority of cases, despite advancements in treatment modalities, including chemotherapy, targeted therapy, and immunotherapy. The prognosis for NSCLC patients remains poor, largely due to challenges such as drug resistance and relapse. This study investigates the role of microRNAs (miRNAs) in the apoptotic pathways associated with NSCLC, focusing specifically on miR-142 and miR-195. These miRNAs have been implicated in regulating critical cellular processes, including proliferation and apoptosis.

This study employed 5-fluorouracil (5-FU), a common chemotherapeutic agent, to assess its cytotoxic effects on the A549 NSCLC cell line. The half-maximal inhibitory concentration (IC50) for 5-FU was determined to be 10.84 µg/mL after 48 hours. Morphological studies revealed significant changes in cell structure post-treatment, while flow cytometry indicated that 31.3% of cells underwent early apoptosis. Quantitative real-time PCR (qRT-PCR) analyses demonstrated a significant upregulation of p53 and Bax, alongside a downregulation of Bcl-2 in treated cells, suggesting a shift towards pro-apoptotic signaling. Notably, miR-142 expression was significantly increased, while miR-195 showed a non-significant upward trend.

These findings underscore the potential of miRNAs as therapeutic targets in NSCLC, particularly in overcoming drug resistance by modulating apoptotic pathways. Further exploration of miRNA-based therapies may yield innovative strategies to enhance treatment efficacy and improve patient outcomes in lung cancer.

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Published

2025-03-04

How to Cite

Davarnia, A., Kharratian, Y., & Nourani, M. R. (2025). The Role of miR-142 and miR-195 in Regulating Apoptotic Pathways in Non-Small Cell Lung Cancer. Life Sciences Student Journal, 3(1), 38–53. https://doi.org/10.22034/LSSJ.2025.130

Issue

Vol. 3 No. 1 (2025): Vol.3 No.1 (2025)

Section

Articles

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