Immunohistochemical Analysis of F4/80-Positive Macrophages in the Interstitial Tissue of Mouse Testis

Abstract

This research work focuses on the localization and distribution of F4/80-positive macrophages in interstitial tissue from the adult mouse testis with the intent of their involvement in spermatogenesis and testicular homeostasis. Herein, we have stained sections from five male C57BL/6 mice by immunohistochemistry and identified an extraordinary accumulation of F4/80-positive macrophages with irregular shapes displaying a dendritic-like appearance in the interstitial spaces. These cells form a network that allows them to interact with germ cells and Sertoli cells, playing an important role in sperm development and immune functions in this immune-privileged organ. Macrophages may also modulate the function of Leydig cells and thereby testosterone production, underlining their highly versatile roles in male reproductive health. Our findings highlight the possible role of F4/80+ macrophages as a therapeutic target in male infertility and further studies investigating the interaction of these macrophages with testicular cells under various pathological conditions may be required.