The comparison between HIIT and Resistance Training in muscle expression of FTO and PPAR-γ in obese diabetic rats
HIIT vs. Resistance Training: Impact on FTO and PPAR-γ in Obese Diabetic Rats
DOI:
https://doi.org/10.22034/LSSJ.2024.114Keywords:
Aerobic, T2D, Interval, Mice, Glucose, HIIT, FTO, PPARyAbstract
Objective: According to the World Health Organization (WHO), the prevalence of overweight and obesity among adults aged 18 and above is estimated to be around 2 billion worldwide. The purpose of this study was to investigate the comparison between High Intensity Interval Training and Resistance Training in expression of FTO and PPAR-γ genes in muscle tissue among type 2 diabetic obese rats.
Methods: This experimental study utilized a sample of 12 male Wistar rats, with an average bodyweight of 220±20 g and an age of 10 weeks. The rats were subjected to a high-fat diet for a duration of six weeks. To induce type 2 diabetes, a single intraperitoneal injection of 30 mg/kg of freshly prepared streptozotocin (STZ) was administered, dissolved in citrate buffer with a pH of 4.5. The rats with diabetes were then randomly divided into three groups: HIIT, Resistance training. Measurements were taken 48 hours after the final training session. The expression level of FTO and PPAR-γ in the muscles were assessed using the real-time PCR method. Statistical analysis was conducted using independent samples t-test and Analysis of Covariance (ANCOVA) to compare the means between the groups.
Results: FTO expression decreased significantly in the HIIT group (P<0.042) while, the RT group faced no significant changes (P<0.452). However, PPAR-γ expression increased only in the HIIT group (P<0.020). The were no significant differences between the changes of FTO and PPAR-γ expression between groups (FTO P<0.243 and PPAR-γ P<0.158).
Conclusion: the findings from this study suggest that short-term and high-intensity aerobic exercises have a more pronounced impact on the expression of FTO and PPAR-γ genes, as well as related factors, in individuals with obesity and type 2 diabetes compared to resistance exercises. The aerobic exercises demonstrated significant alterations in the expression of both the FTO and PPAR-γ genes.
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