Molecular analysis of the chicken anemia viruses isolated from broiler farms of East Azerbaijan province using PCR-RFLP

Abstract

Anemia was first detected in Japan. Since then, the CAV virus has been reported in broiler and oviparous chicken breeds in many countries, both in viral isolation and serologically. One of the major immunosuppressive viruses is infection anemia virus. Infections with the CAV virus appear to be clinically prominent, including symptoms of bone marrow cells, subcutaneous hemorrhage, atrophy of lymph nodes, and increased mortality. The virus has a circular DNA genome of 2,300 bp, including three protein sequences (VP1, VP2, and VP3). The 50 kDa (VP1) viral protein is the only isolated protein from viral objects. The study examined the prevalence, traceability, isolation and identification of an infective anemia virus in broiler flocks within East Azarbaijan province. For this purpose, 100 samples of slaughtered broiler flocks were collected at slaughterhouses in the East Azerbaijan province. To confirm the presence of CAV virus in the samples, PCR was performed and the results were studied with RFLP technique. This study showed that poultry infectious anemia virus in broiler farms in East Azarbaijan province is widespread. There are very few differences in virus between isolates and isolates from various parts of the world.