Cytotoxic effects of dimethyl-celecoxib (DMC) on bone tumor cell line MG63 and cell line HEK293
DOI:
https://doi.org/10.22034/JATE.2023.86Keywords:
Bone tumor, Dimethyl-celecoxib, HEK293, MG63Abstract
Purpose: Dimethyl-celecoxib (DMC), is a celecoxib (CXB) derivative has no inhibitory function on cyclooxygenase-2 (COX-2) and displays antitumor properties. This substance can be helpful in advancing the treatment of COX-2-indipentent cancers. In this study, we assayed the efficacy of DMC on MG63 bone tumor and Human embryonic kidney (HEK293) cell lines.
Methods: The cellular viability, nitric oxide content, and Inducible nitric oxide synthases (iNOS) gene expression were measured respectively with MTT (The MTT assay is a colorimetric assay for assessing cell metabolic activity.) Griess reaction, and real-time Reverse transcription polymerase chain reaction (RT-PCR) procedures. IC50 was determined by MTT assay, and iNOS gene expression was evaluated by RT-PCR. Also, monoxide nitrogen production was monitored by a Griess test and finally one-way Anova (Analysis of Variance (ANOVA) is a statistical formula used to compare variances across the means (or average) of different groups.) and T-test were used to analyze the data.
Results: The Results clearly showed that Dimethylcelloxib at concentrations of: 62.5, 125, 250, 500 μg /ml at 48 h significantly decreased the survival rate of MG63 and HEK293 tumor cells (P<0.001). It was found that iNOS gene expression decreased significantly (P<0.001) and production of monoxide nitrogen molecule had a significant increase (P<0.01 and P<0.001). Conclusion: Our research showed that Since Dimethylceloxib reduced iNOS gene expression, it is expected to decrease nitrogen monoxide, but this drug showed its cytotoxic effects through increased nitrogen monoxide production.
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