Investigating the expression level of VEGF and FLT 1 genes in wound healing using electrospun multilayer scaffold in animal model
DOI:
https://doi.org/10.22034/JATE.2026.112Keywords:
Keywords: Electrospinning, biological nanoglass, collagen generation, wound healing and gene expressionAbstract
Background and purpose:
Treating skin lesions with bioscaffolds has been a new step in biotechnology and medical studies in recent years. Due to the multi-layered structure of skin tissue, the use of a three-layer scaffold made using an electrospinning technique can lead to planned and targeted skin regeneration. The purpose of this research is to investigate the role of effective VEGF expression and collagen production in healing full-thickness wounds using a three-layer scaffold that has a structure imitating skin tissue and can reduce the duration of wound healing.
Materials and methods:
Here, the physical properties of the scaffold were investigated using XRD. Also, after L929 fibroblasts were seeded, cellular scaffolds were implanted in full thickness wounds of rats, and the degree of wound closure was evaluated using histological and genetic techniques.
Findings:
The evaluations performed on the scaffold confirmed the physical characteristics. In vivo studies showed better wound closure with scaffolds containing zinc ions. This scaffold also showed better observations with histological evaluations than nontreated and control groups. Real-time PCR confirmed the higher expression of VEGF and FlT1 genes in mice treated with scaffolds containing zinc ions.
Conclusion:
Scaffolds with bio-nano glass, whether 45s5 type or type containing zinc ions, showed a better ability to heal skin injuries.
References
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